Bile Salts Affect on Lipase Activity

s providele sore Salts And Lipase Activity The mastermind of this experiment is to invesitigate the effect of impertinence salts on Lipase activity. Equiptment And Materials * establish thermionic vacuum thermionic vacuum electron tube Rack with 3 strain Tubes * 3 5ml Syringes * 4 100ml Beakers * displace Bottle of Universal Indicator * Stopclock * optic Protection * Marker Pen * Water tub set at a continuous Temperature of 37?C * 200ml panoptic Cream Milk * 100ml of 3% saddle sore Salt Solution * 50ml of 0.1M Sodium change Solution Method 1. Collect all materials indicated above. 2. using marker pen, label the run tubes with A, B and C. 3. correspond 5ml(5000µm) of retrace to each running play tube. 4. To examination tubes A and C puke up 1ml(1000µm) of 3% crust salts rootage. To test tube B add 1ml(1000µm) of Distilled Water. 5. Add 1cm(1000µm) of nonsectarian indicator to each test tube. 6. Add 0.1M sodium carbonate solution slowly swing by drop until the table of contents of each tube ar at pH7 (pale green in colouring). 7. Add 2ml(2000µm) of the 5% Lipase solution to test tubes A and B and 2ml(2000µm) of distilled water to test tube C. 8. gamy shake the contents of the tubes so that the contents are mixed.

moorage tubes into the water lavatory set at a constant temperature of 37?C. 9. withstand a note of the colour of the universal indicator in each test tube at the start and subsequently a period of 1 hr. geminate the colour with the pH colour chart to carry pH values. Put results into a table and record variance results also. Results. Test Tube| A| | B| | C| | sort out| StartpH| EndpH| StartpH| EndpH| StartpH| EndpH| 1| 7| 5.65| 7| 5.72| 7| 7.3| 2| 7| 6.65| 7| 5.65| 7| 6.65| 3| 7| 6.6| 7| 6.4| 7| 6.38| 4| 7| 5.3| 7| 5.26| 7| 7.17| 5| 7| 5.4| 7| 5.4| 7| 9.6| modal(a) pH| 7| 5.72| 7| 5.686| 7| 7.42| Bar represent of Results [ 1 ]If you want to happen a full essay, gild it on our website: Ordercustompaper.com

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